Zhuge Hormin wrote , ” Traditional PCR reactions require temperature-changing steps, so they rely on expensive PCR machines. Although the existing isothermal amplification technology uses simple equipment, it cannot replace PCR. Published in “Nature Communications” on the 23rd of this month Isothermal amplification PCR was taken to a new level in a study by Isothermal Amplification that reengineered a helicase (PcrA M6) from their previous study and increased its activity by a factor of 4, thus replacing the DNA temperature denaturation step, allowing Isothermal amplification PCR becomes possible. The experimental results show almost the same characteristics and effects as traditional PCR. For example, only two primers are used, the time is short, and the plasmids and fragments in the genome can be amplified. The method is named SHARP (SSB- Helicase Assisted Rapid PCR).
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